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Impact of antidiabetic substances to development of insulin resistance and neurodegenerative changes in mouse models of type 2 diabetes
Mikulášková, Barbora ; Čabala, Radomír (advisor) ; Kuneš, Jaroslav (referee)
Numerous epidemiological and experimental studies have shown that patients suffering from metabolic disorders such as type 2 diabetes mellitus (TDM2), insulin resistance or obesity are at a higher risk of cognitive functions impairment and developing Alzheimer's disease (AD). Impairment of insulin signalling in the brain could contribute to two pathological changes which leads to AD development that include insoluble senile plaques and neurofibrillary tangles, containing an abnormally hyperphosphorylated tau protein (Tau). This work is focused on investigating of insulin signaling in hippocampi in the brains of mice models of insulin resistence, impact of disturbed insulin signaling on hyperphosphorylation of Tau, and possible benefical efect of insulin sensitizing agents on insulin signaling and Tau phosphorylation in the hippocampi of diabetic mice. The first, we examined insulin signaling and phosphorylation of Tau in hippocampi in two mouse models of TDM2 - lipodystrofic A-ZIP F-1 mice and monosodium glutamate obese mice (MSG mice). We did not observe any changes in insulin signaling and Tau phosphorylation in hippocampi of A-ZIP F-1 mice compared to controls. In the hippocampi of MSG mice there was attenuated phosphorylation of kinases of insulin signalling including Ser9 of glycogen synthase...
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Impact of antidiabetic substances to development of insulin resistance and neurodegenerative changes in mouse models of type 2 diabetes
Mikulášková, Barbora ; Čabala, Radomír (advisor) ; Kuneš, Jaroslav (referee)
Numerous epidemiological and experimental studies have shown that patients suffering from metabolic disorders such as type 2 diabetes mellitus (TDM2), insulin resistance or obesity are at a higher risk of cognitive functions impairment and developing Alzheimer's disease (AD). Impairment of insulin signalling in the brain could contribute to two pathological changes which leads to AD development that include insoluble senile plaques and neurofibrillary tangles, containing an abnormally hyperphosphorylated tau protein (Tau). This work is focused on investigating of insulin signaling in hippocampi in the brains of mice models of insulin resistence, impact of disturbed insulin signaling on hyperphosphorylation of Tau, and possible benefical efect of insulin sensitizing agents on insulin signaling and Tau phosphorylation in the hippocampi of diabetic mice. The first, we examined insulin signaling and phosphorylation of Tau in hippocampi in two mouse models of TDM2 - lipodystrofic A-ZIP F-1 mice and monosodium glutamate obese mice (MSG mice). We did not observe any changes in insulin signaling and Tau phosphorylation in hippocampi of A-ZIP F-1 mice compared to controls. In the hippocampi of MSG mice there was attenuated phosphorylation of kinases of insulin signalling including Ser9 of glycogen synthase...
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A type 2 diabetes nutrition - recommendation and reality
MÍČKOVÁ, Veronika
The given bachelor thesis elaborates on the issue of type 2 diabetes and the approach of the patients, suitability of their education about the recommended diet and formation of potential risks as a result of this disease. Two main objectives were set for this study. The first objective consisted of a survey on education of the patients about the recommended diet and health risks if they fail its observance. The second objective analysed the survey which was conducted in order to reveal factual eating habits of these patients. The first and purely theoretical part. The second part focuses on qualitative research which was conducted by using a semi-structured interview as a data collection method. The focus group consisted of six respondents - two men and four women. The gathered facts reveal that the education and knowledge instructed by the doctor is more or less satisfactory. Four respondents out of six indicated to be satisfied with their doctor. Only one respondent was utterly unsatisfied and one assessment provided by a female respondent was considered as biased considering her old age and a long course of the illness. Her negative approach towards the doctor´s education was not taken into account either, due to the fact that the given patient must have changed several doctors during her lifetime. The results also demonstrate that the majority of respondents observe a recommended diabetes diet, eating at least five or six meals a day. According to their answers, most of them eat vegetables, lean meat and whole-grain bread and drink water, tea, or coffee. Five respondents out of six prioritise the recommended food preparation techniques, such as steaming, stir-frying and stewing. They all agree on types of food they try to avoid in majority sweets. Then the conducted survey points out situations during which the given diet is not observed. However, these situations are exceptional, so they should not be regarded as a non-observance of the diet provided that the answers given are truthful. Furthermore, the respondents observe, or try to observe, the diet recommendations, as evidenced by their regular doctor´s appointment and steady glycaemic index. Finally, the given research included two questions which were supposed to highlight the possibilities of the respondents depending on their place of residence. The first question should indicate the variation in size of the place and number of inhabitants. It was proved that the size did not have a remarkable influence on the diabetes education. The second question focused on assortment and price of diabetes food products. The bigger the agglomeration is, the cheaper and wider assortment of these products can be found. The final assessment based on interviews and results obtained was more or less positive and in comparison to other publications specifying the same recommended diet reached similar conclusions.
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